There are many types of special equipment that is used in medical centers. One of these is the Elisa kit. This is a piece of equipment that is used for the purpose of detecting the presence or absence of antigens or antibodies in the blood.
ELISA stands for Enzyme Linked Immunosorbent Assay. This kit is utilized in medical centers to detect antigens, peptides and proteins. They are also used to detect EGF, VEGF as well as cytokines. The Elisa tests are designed for each specific species. Some of the species for which Elisa tests have been developed include.
- Ovine or sheep species
- Caprine or goat
- Feline or cat
- Bovine or cow
The pathogens that can attack one species are different from those that can attack another. As such, each species needs its own special diagnosis equipment. For this reason, manufacturers such as MyBioSource develop a variety of Elisa tests for the different species of animals across the world. If you don’t find a kit for a specific species, you can simply order a custom made kit from the manufacturers.
Why are these test kits important to scientists and doctors?
Elisa test kits are used for far more than just detecting antibodies. They can also be used to determine pathways for biochemical materials in the body. These test kits are used to detect the pathway that is used for signaling insulin in the body. They are also used to detect the pathway that is used in the process of oxidative stress.
These test kits are also used for analyzing the various states of diseases. For example, they are used extensively in studying the various stages and states of cancer. As a result, they play a major part in the diagnosis and management of the disease.
Elisa kits are designed such that they can accept various types of sample materials. They are effective in detecting antibodies in the following materials;
- Tissue homogenates
- Lysates from cell cultures
To make the experience of the scientists and doctors more convenient, the test kits are designed such that they have various ranges of detection. They also have different levels of sensitivity to the antibodies. This provides the doctors with convenience as they work. There are some outstanding designs of these kits.
Some Elisa kit designs
One of these designs is the competitive Elisa test kits. In this design, the kit has a labeled antigen that works to compete with the antigens in the sample that you place. These antigens compete to bind with the capture antibody and allow the laboratory technician or scientist to read results.
Another design is where the plates of the kit are coated with an antigen. The technician simply adds the antibodies and an enzyme. The mixture reacts and they are able to read results and tell if the patient is infected or not.
Elisa kits are especially important to scientists and doctors all over the world. They are an effective and convenient testing mechanism. Health and safety standards should always be observed when working with these kits.
Sammy Bolder is a laboratory technician at a leading pharmaceutical corporation. He has decades of experience performing his job with Elisa test kits.